Rapid confocal imaging of large areas of excised tissue with strip mosaicing

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Rapid confocal imaging of large areas of excised tissue with strip mosaicing.

Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and processing times, and produces artifacts. To reduce both time and artifacts, ...

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Confocal microscopy with strip mosaicing for rapid imaging over large areas of excised tissue.

Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in freshly excised tissue, without the processing required for conventional pathology. Previously, mosaicing on 12-×-12  mm² of excised skin tissue from Mohs surgery and detection of basal cell carcinoma margins was demonstrated in 9 min. Last year, we reported the feasibility of a faster approa...

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Fibered confocal microscopy allows in vivo and in situ imaging with cellular resolution. The potentiality of this imaging modality is extended in this work by using video mosaicing techniques. Two novelties are introduced. A robust estimator based on statistics for Riemannian manifolds is developed to find a globally consistent mapping of the input frames to a common coordinate system. A mosaic...

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ژورنال

عنوان ژورنال: Journal of Biomedical Optics

سال: 2011

ISSN: 1083-3668

DOI: 10.1117/1.3582335